The National Programme on Conservation and Utilization of Microbial Genetic Resources and Invertebrates of Agricultural Importance

COLLECTIONS OF BACTERIA

VURV–B VURV–R RIBM RIFIS CAPM CCDM CCDBC CCM

Collection of Phytopathogenic and Agriculturally Beneficial Bacteria (VURV–B), (CARC, Prague)

Collection of phytopathogenic and agriculturally beneficial bacteria (CPABB) is one of the founding collections of The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture. The collection currently includes:
1) Strains of quarantine bacterial plant pathogens and other plant pathogenic bacteria causing severe extensive damage to field and greenhouse crops, to forests, orchards and ornamental plants.
2) Strains of agriculturally beneficial bacteria supporting growth and viability of plants and concomitant bacteria which demonstrate properties of biotechnological values for agricultural research and practice.
Collection of phytopathogenic and agriculturally beneficial bacteria is administrated and maintained by staffs of team of Phytobacteriology, the only workplace in the Czech Republic specializing in bacterial plant diseases. Our laboratory, greenhouse and experimental plots in Slaný have a working permit to handle group 2 microorganisms.
The Plant-Pathogenic Bacteria Database includes 270 strains of 19 genera and 44 species: https://www.vurv.cz/collections/vurv.exe/list?lang=cz&org=BA&term=&coll={9ABB1809-129E-4165-AF01-60B5EB319A25}&cond=AND&rows=20&item_no=1
Agrobacterium, Bacillus, Clavibacter, Curtobacterium, Dickeya, Erwinia, Flavobacterium, Leifsonia, Microbacterium, Mycobacterium, Paenibacillus, Pantoea, Pectobacterium, Pseudomonas, Ralstonia, Rhizobium, Stenotrophomonas, Streptomyces, Xanthomonas

• Activities
Monitoring of causal agents of bacterial diseases and other accompanying bacteria important for the agricultural crops production and food processing industry, orchards, ornamental horticulture and forests in the Czech Republic.
Epidemiology and genetic variability of plant pathogenic bacteria.
Determination of characteristic features of individual strains of bacteria - microscopic, biochemical, genetic and chemical, virulence and pathogenicity and another specific properties - surface tension, ice nucleation activity, antagonistic properties, resistance to antimicrobial chemical agents.
Deposition and maintaining of plant pathogenic bacterial strains.
Online providing evidence of the deposited strains of bacteria and tables summarizing their characteristics.

• Services
Providing deposited bacterial strains with guaranteed characteristics in the required quality and quantity.
Reference strains for diagnostic laboratories.
Rapid identification services based on automated fatty acid profilig - FAME, characterisation methods - including nutritional profilig - Biolog, genetic profilig – real-time PCR, HRM.
Expert activities - diagnostics of causal agent of bacterial diseases of plants and bacterial contaminants in the processing of agricultural crops in food technology.
Training and educational activities - for civil servants, agricultural companies, diagnostic laboratories, high schools, universities, students and professionals.
Teaching – lectures for middle and high schools, assistance in the preparation of thesis or dissertation.
International exchange - research centres, universities.
Depositing of new strains important for agricultural as well as basic and applied researches after the acceptance process.

• Preservation
Most of the bacterial strains are cryopreserved at - 90°C.
Major part of deposited strains is freeze dried and stored at room temperature.
All cultures delivered by CPPB are for immediate use – particularly as actively growing cultures on nutrient agar plates or slants.
DNAs are stored in TE buffer at -30°C and are sent particularly at room temperature.
All strains are checked after preservation for viability, purity, virulence and pathogenicity after intervals scheduled for the different taxa.
Bacterial strains provided for deposit are tested by subculturing at least once a year.

Application:
• Contact and information for customers
All strains are supplied within 2-4 weeks according type and number of strains.
Terms and conditions form will be send to customer before expedition.
The only cost would be the shipping charges.
Customer agrees to cite the CPPB strain number(s) in publications and applications and is kindly requested to provide the CPPB with details of the related publications.

Contact:
Czech Agrifood Research Center
Team of Phytobacteriology
Drnovská 507
161 06 Praha 6 – Ruzyně
Czech Republic
https://www.carc.cz

Staff:
Ing. Iveta Pánková, Ph.D. - pankovai@vurv.cz, 702 087 647, 233 022 289 (responsible person)
Ing. Václav Krejzar, Ph.D. - krejzar@vurv.cz, 702 087 648, 233 022 470
Ing. Radka Krejzarová, Ph.D., Ing. Barbora Soukupová

Collection of Soil Bacteria (VURV–R), (CARC, Prague)

Culture collection of soil microorganisms is maintained since 1948 .In the last 20 years the collection has been directed to specialize in bacteria cultures of Rhizobium, Bradyrhizobium and Sinorhizobium spp.Culture collection is enriched by several strains of the Azotobacter spp.
The collection of tropical and subtropical rhizobia of the Czech University of Life Sciences Prague (Institute of Tropical and Subtropical Agriculture) was added to the Collection of Rhizobia in 1996.
Rhizobia are bakteria able to fix atmospheric nitrogen in symbiosis with legumes, Azotobacter spp. are beneficial free-living nitrogen fixers.

• Characterization of collection
Presently the collection contains 510 strains of Rhizobium spp.and 28 strains of Azotobacter spp.

• Methods of culture maintenance
Cultures are maintained both on the pea agar slants (or YEM agar slants) and as freeze dried.
Besides the maintenance of cultures in the collection isolations of new rhizobia strains from legume plants cultivated under field condition are realized. All the microbiological works are practised under sterile conditions in the laboratory or in the flow-box. The usual working methods and safety rules are adhered.

• Activities, services
Research activities are focused in identifying of nitrogenase aktivity and effectiveness of different rhizobial strains in symbiosis with selected species of legumes. Resarch of Azotobacter spp. is directed at usability in the soil inoculations and increasing the soil fertility. The catalogue is published 1x 3 years.

Application:
Cultures Collection of Rhizobia is a member of Federation of Czech and Slovak Culture Collections (FCCM) since 1969.
One hundred of selected strains is registered in World Catalogue of Rhizobium Collections, (F.A.Skiner, E.Hamatová, V.McGovan: World Catalogue of Rhizobium Collection, ed. V.B.D.Skerman, 1983, FAO/UNESCO).
Cultures Collection of Rhizobia serve for institutions dealing with symbiotic nitrogen fixation.
In the Department of Soil Biology, strains from collection are used for research by T.Šimon and O. Mikanová. Strains are cultivated also for other department and research stations of Czech Agrifood Research Center. The collection supplies cultures for scientiffic and pedagogical purposes.
There are assistance aktivity and consultancy for the Czech company producing inoculants and biofertilizers. (Farma Žiro, s.r.o., Nehvizdy).

Contact:
Culture Collection of Rhizobia is maintained in Division of Crop Managmenet Systems, Agricultural Soil Science and Pedobiology in Czech Agrifood Research Center in Prague – Ruzyně, Drnovská 507.

Staff:
RNDr. Veronika Řezáčová, Ph.D. rezacova@vurv.cz 233 022 308 (curator)
Ing.Tomáš Šimon, CSc. simont@vurv.cz 233 022 253

Collection of Brewery Microorganisms (RIBM), (VÚPS, a. s., Praha)

The collection of brewer's yeast was established in 1946 as part of a collection of yeasts and yeast-like microorganisms under the leadership of Dr. Kocková-Kratochvílová, and has operated independently since 1953. Since 1964 the collection has been a member of the Federation of Czech and Slovak Collections of Microorganisms and is internationally registered as RIBM under the number 655. By focusing on the production strains of brewing yeast it is unique in Central and Eastern Europe. In 1996 the brewer's yeast collection became (as one of very specifically targeted and industrially applicable collections) part of the "National Program of the Gene Pool of Microorganisms and Small Animals of Economic Importance and their Use in Reference Diagnostics." This project is also supported by the Czech government in the form of a grant by the Ministry of Agriculture. The program is led by the Council of Microbial Genetic Resources, which monitors the mandatory retention of a functional existence of collections of microorganisms of a corresponding character.

• Characterization of collection
RIBM collection includes two separate collections, collections of brewer's yeast and collection of bacteria and wild and wine yeast. The collection currently includes a total of 308 strains of yeasts and bacteria. The most important part of the collection consists of a collection of 118 strains of brewer´s yeast Saccharomyces cerevisiae and S. pastorianus, collected continuously since 1953 from Czech and foreign breweries. A collection of wild and wine yeasts contains 79 strains classified into genera Saccharomyces, Torulaspora, Zygosaccharomyces, Dekkera, Williopsis, Pichia, Schizosaccharomyces, Saccharomycodes, Candida, Kloeckera and Rhodotorula. Collection of bacteria houses 108 strains of Lactobacillus, Leuconostoc, Pediococcus, Tetragenococcus, Lactococcus and three strains of genus Pectinatus.

• Methods of culture maintenance
Collections of brewing and wild yeasts are kept on agar slants under paraffin-covered cotton wool plugs and simultaneously on wort agars overlayered with sterile paraffin oil separately in a refrigerator. These best ways of keeping the cultures allow the rapid delivery to a customer of a strain in an active state on an agar slant or fermenting in 1.5 liters of wort, which facilitates the transfer of the production strain into production. Since 2006 strains of brewer's yeast are stored in cryo-tubes with protective medium in liquid nitrogen at -196 ° C. Storing in liquid nitrogen (cryopreservation) is considered optimal for the long term preservation of yeast in a viable state. Lactic acid bacteria are stored in parallel freeze-dried in skimmed milk in liquid nitrogen.

Application:
Use of the collection:
Collection strains are primarily used for research projects of RIBM and other research organizations (MSM6019369701, 1M0570, 2B08022 2A-2TP1 / 031, FI-IM5 / 067, QF3299, QI91B226 etc.). The strains are also used for teaching and diploma and doctoral works at universities.

Contact:
Research Institute of Brewing and Malting
Lípová 15
120 44 Prague 2

• curator
Ing. Petra Kubizniaková
kubizniakova@beerresearch.cz
phone.: 224 900 152

• others
RNDr. Dagmar Matoulková, Ph.D.
matoulkova@beerresearch.cz
phone.: 224 900 132

WWW: http://www.beerresearch.cz

Collection of Industrially Utilizable Microorganisms (RIFIS), (CARC, v. v. i.)

• Activities, research activities, services
Czech Agrifood Research Center, v.v.i. manages and keeps the unique collection of industrially useful microorganisms RIFIS. The collection serves the needs of agriculture and food industries. The strains are used for processing food and agricultural commodities, for control analytical activities and research tasks solved in CARC, v.v.i. and elsewhere. We provide deposited bacterial strains with guaranteed characteristics in the required quality and quantity for scientific and research purposes.

• Collection characteristics (strains), culture counts
The collection contains bacteria, yeasts, and molds important in the food and agriculture industries. In 2023, the total number of strains was changed to 155, including 135 yeast strains, 13 bacterial strains, and 7 mold strains.

• Methods of strain preservation
The strains are preserved on slanted agar in an active state and stored in a refrigerator at 4 - 7 °C. Since 2020, strains have also been stored in cryovials with protective media in a deeply frozen state at a temperature of -80°C. Bacterial strains are also long-term preserved by lyophilization method, which is performed externally at the central laboratory of NPGZM at the VURV v.v.i. workplace using the large-capacity lyophilizer Triad Cascade Benchtop Freeze Dry systems from Labconco.

• Utilization of the collection
Collection utilization: Utilization of the collection: • Utilization of the strains The RIFIS collection contains 13 bacterial strains, including Lactobacillus, Bacillus, Alcaligenes, and Pseudomonas. The collection includes strains that can be used for microbiological analytical methods. It also contains strains that can be used for the remediation of oil substances, lactic acid bacteria, and probiotic strains. Additionally, the collection contains a strain that produces the enzyme cyclodextrin glucosyltransferase. Some strains are common contaminants of food.

• Users and clients
The primary users of the collection are research institutions working in the field of microbiology, food production, and agriculture. Some examples of clients include the Laboratory of Enzyme Technologies at the Institute of Microbiology of the Czech Academy of Sciences in Prague, the Laboratory of Biology and Microbiology at the Institute of Landscape Ecology of the Czech Academy of Sciences in Ceske Budejovice, the Research Institute of Brewing and Malting, the Institute of Antibiotics and Biotransformation in Prague, and others.

Responsible researcher/curator: Mgr. Radko Pechar, Ph.D.
tel.: +420 775435055
e-mail: radko.pechar@carc.cz
Other researcher: Ing. Marian Urban, Ph.D.
phone: 737 287 003
marian.urban@carc.cz
Ing. Zdeněk Moučka
phone: 737 287 009
zdenek.moucka@carc.cz

fermentor

strains on sloping agars

Sacharomyces cerevisiae

Y lipolytica Candida sp. lipase detection

Collection of Animal Pathogenic Microorganisms (CAPM),
(VÚVeL, v. v. i., Brno)

The Collection of Animal Pathogenic Microorganisms (CAPM) was founded in 1962. At present the CAPM is a part of the Department of Bacteriology at the Veterinary Research Institute in Brno.

• Characterization of collection
The major activities of the CAPM involve acquisition, preservation and distribution of microorganisms - animal viruses and zoopathogenic bacteria useful for veterinary practice, applied microbiology and research. The CAPM holds more than 300 strains of viruses and almost 600 strains of bacteria which are listed in printed catalogues.

• Activities, services
The main function of the CAPM is to collect, maintain, preserve and supply pure cultures of microorganisms. The CAPM accept for deposit viral and bacterial strains which are subject to patent applications on the national level.

• Other services:
Safe deposit of cultures
Freeze-drying of cultures
Consultation

• Preservation
Cultures are preserved by freeze-drying or freezing in liquid nitrogen or kept at -80 °C.

• System of quality
Laboratory works are carried out in compliance with the approved methodology (“Standard procedure for preservation, storing, characterization and documentation of genetic resources”), and the principles of Good Laboratory Practice (GLP) are being observed in the workplace.

• Membership
The CAPM is a member of the World Federation for Culture Collections - WFCC , the European Culture Collections' Organisation - ECCO and the Federation of Czech and Slovak Culture Collections - FCCM .

Application:
• Application of strains
Viral and bacterial strains are predominantly used for research, diagnostic and educational purposes. Besides agriculture, education and health care, they can be also employed in biotechnology and in industry.

• Customers
CAPM cultures are supplied to laboratories, schools and educational institutions both in Czech Republic and other countries.

Contact:

Veterinary Research Institute
Hudcova 70, 621 00 Brno
Czech Republic
Telephone 533 332 131
Fax 541 211 229
WWW www.vri.cz

Staff
MVDr. Markéta Reichelová - Head, curator (bacteria)
reichelova@vri.cz

Other staff:
Mgr. Hana Malenovská - curator (viruses)
malenovska@vri.cz

Culture Collection of Dairy Microorganisms Laktoflora® (CCDM), (Milcom, a. s., Tábor)

The Culture Collection of Dairy Microorganisms, Laktoflora® (CCDM) is a unique collection of bacterial cultures (monocultures and mixed cultures) and fungal organisms. The collection was established in 1956 in Prague and is currently located at the workplace of the Dairy Research Institute in Tábor (MILCOM a.s.). In 2021, a total of 974 strains were registered in the Laktoflora® collection. Most bacterial cultures are stored in a lyophilized state and at the same time deep-freezed by cryopreservation. Yeasts, moulds, and some bacterial strains are kept on nutrient agar medium and are stored by cryopreservation. The collection is significantly funded by National Program for the Conservation and Utilization of Genetic Resources of Microorganisms and Small Animals of Economic Importance (NPGZM). Since 2019, the CCDM collection includes the so-called bakery collection consisted of bacteria and yeasts with specific technological properties predominantly isolated from different kinds of sourdough and used in the bakery industry.

• Collection characteristics, numbers of cultures
In 2021, a total of 810 bacterial strains are in the collection. Most strains (741) are lactic acid bacteria (monocultures-580, mixed cultures-161). Monocultures of lactic acid bacteria are mainly represented by the genera of Lactobacillus, Bifidobacterium, Enterococcus, Lactococcus, Streptococcus and Propionibacterium. Mixed cultures mainly include yoghurt and starter cultures. The existing CCDM collection will be extended in the future with new isolates with functional properties corresponding to current trends and requirements of the dairy industry. The new collection of bakery strains contains 46 bacterial strains and will continue to expand.

• Methods of storage
Three methods of storage are used:
lyophilization: long-term storage of lactic acid bacteria; recovery interval 5-8 years
slant agar: short-term storage of surface-growing strains, annual reinoculation
cryopreservation: all bacterial strains; recovery interval 5 years

• Quality systems, internal standards, good laboratory practice
The activity of the CCDM collection is defined by the Directive for the management of the activity of the collection according to ISO 9001. Based on the control audits, the Laktoflora® collection was certificated in 2002. The operations of the collection are further ensured in accordance with the internal guidelines. All methods of deposition are described in managed documents.

• Services
all the strains of the CCDM collection are provided free of charge for the teaching and research institution in accordance with the national program (NPGZM)
the service of isolation, cultivation and identification of strains is provided
long-term deposition of your strains into the collection
professional advisory and consulting activities

• National and international organizations
The CCDM collection is a part of the National Program for the Conservation and Utilization of Genetic Resources of Plants, Animals and Microorganisms Important for Food and Agriculture (NPGZM). Worldwide, the CCDM collection cooperates with the collections of CBS, Westerdijk Institute (Netherlands), MIRRI (France) and CCY (Slovakia). The CCDM collection is registered in the National Library of Medicine Database Maintenance Project and in the World Data Center for Microorganisms (WFCC 874).

Contact:

Culture Collection of Dairy Microorganisms Laktoflora®
MILCOM a.s., Soběslavská 841, 390 02 Tábor, Czech Republic
phone: +420 733 591 080, www.ccdm.cz

Ing. Miloslava Kavková, Ph.D., the head of the collection CCDM
m.kavkova@vum-tabor.cz
+420 723 679 012

Mgr. Ladislav Bár, the curator of the collection CCDM
l.bar@vum-tabor.cz
+420 733 591 080

Closing the vials before lyophilization

Lyophilizer Cryodos 50

Storage of lyophilized cultures

Microscope Olympus BX-43

Microscopic control of cultures

Thermal cycler Biometra

Biohazard flow box

Cryopreservation in the freezer

Cultures in broths and in lyophilized form

Czech Collection of Microorganisms (CCM), (MU, Brno)

The Czech Collection of Microorganisms (CCM) is a nonprofit organization established at the Faculty of Science, Masaryk University in 1963 and in the present serves as a specialized research and service division within the Department of Experimental Biology. The CCM was included into the National Programme (NPGZM) in 2019 with selected collection of approx. 200 bacterial strains in the frame of the project: „Microorganisms related to dairy technologies and products“.

• Characterisation of the CCM
The CCM serves as a depository for cultures of bacteria and filamentous fungi. The main goal of the CCM is to collect, maintain and preserve authentic cultures of living bacteria and filamentous fungi relevant to applied microbiology, biotechnology, education and those of general scientific interest A special set of strains is used as reference cultures for clinical, veterinary, food and water testing laboratories. The CCM public collection contains about 3400 bacterial strains covering more than 1700 species and about 800 fungal strains with more than 550 species. All cultures are available via a public catalogue of strains (www.sci.muni.cz/ccm). The CCM also maintains a unique collection of aquatic hyphomycetes containing approx. 500 strains (60 genera and 130 species) and a collection of staphylococcal bacteriophages and their propagation strains which was established in 2011. Since 1992, the CCM is an International Depositary Authority (IDA) for deposits of bacteria, yeasts and filamentous fungi for patent purposes under the Budapest Treaty. There are more than 200 patent strains of bacteria and fungi maintained in the CCM at present.

• Research activities
The major activities of the CCM focus on the preservation and distribution of microorganisms. The CCM continuously extents and diversifies its holdings (approx. 100 cultures per year) and contributes to the biodiversity conservation ex situ. Research activities funded in the frame of national research programmes are focused on the taxonomy of Gram-positive cocci and rods (mainly the phylum Firmicutes), Gram-negative rods (mainly the phyla Proteobacteria and Bacteroidetes) and aquatic hyphomycetes. Since 2008, the CCM has been actively involved in the microbiological exploration of Antarctic environment.

• Services
Cultures supply - CCM cultures are used for basic and applied research, industrial applications, education and general scientific interest. The cultures are distributed under the conditions of the CCM Material Transfer Agreement (MTA) following the OECD Best Practice Guidelines for BRCs and WFCC Culture Collections Guidelines. Holdings - the CCM holds strains of bacteria, fungi and bacteriophages in pure cultures, covering a wide range of applications (type or ex- type cultures derived from type, quality control strains for testing of media, assay of antibiotics, vitamins and amino acids, testing of disinfectants, commercial diagnostic kits, enzymes and secondary metabolites producing strains, biodegrading strains for biotechnology, etc.). Safe keeping - the CCM offers a long-term preservation of viable and genetically unaltered customers' bacterial and fungal cultures under optimal conditions. Deposited cultures remain the property of the depositor. The CCM does not provide these cultures nor any information about them to third parties without the owner's written permission. Freeze-drying - the CCM is able to prepare freeze-dried ampoules to customers who require keeping their cultures for a long time in their own laboratory. Identification of microorganisms - the CCM offers isolation and purification of cultures from provided samples and identification of bacteria and fungi by using phenotypic and molecular techniques. Consultancy – the CCM provides advisory and consultation services on enrichment, isolation, identification and preservation of microorganisms.

• Maintenance of CCM cultures
CCM cultures are preserved mainly as freeze-dried conserves (lyophilisation), also frozen in liquid nitrogen at -196 °C and deep-frozen at -70 °C.

• International cooperation
The CCM cooperates with other culture collections within the Czech Republic as well as abroad. It is recognized by the World Federation for Culture Collections (member No. 65) and is also a member of the European Culture Collections’ Organisation and the Federation of Czech and Slovak Culture Collections.

• Quality system and in-house rules
The CCM introduced the System of Quality Management and was certified according to the ČSN EN ISO 9001:2001 standards on June 22, 2006. Recertification according to the ČSN EN ISO 9001:2015 has been validated since June 4, 2017. All laboratory procedures follow the standard operation procedures complying with the Good Laboratory Practice rules. All information concerning CCM strains and their preservation and distribution is unified in a database based on the MINE (Microbial Information Network Europe) format.

Application of CCM cultures, users
CCM strains are used primarily for the diagnostic purposes as well as for research and education in the Czech Republic or abroad. The most frequent users are public health institutions and laboratories of veterinary medicine but also other research institutions, universities, agricultural laboratories or food and water testing laboratories.

Contact:
Czech Collection of Microorganisms
Department of Experimental Biology
Faculty of Science,
Masaryk University Kamenice 5, buiding A25,
625 00 Brno,
Czech Republic
Tel.: +420-549496922
Web pages https://ccm.sci.muni.cz/

Responsible person:
Prof. RNDr. Ivo Sedláček, CSc.,
Head of the CCM ivo@sci.muni.cz, +420-549496922

Other workers Mgr. Dana Nováková, Ph.D.,
Curator of bacterial collection danan@sci.muni.cz

Ing. Monika Laichmanová, Ph.D.,
Curator of fungal collection monikadr@sci.muni.cz

Culture Collection of Dairy and Bakery Contaminants (CCDBC), (Milcom, a. s., Tábor)

The Collection of Dairy and Bakery Contaminants (CCDBC) was established in 2019 with the support of the Ministry of Agriculture of the Czech Republic as part of the National Program for the Conservation and Utilization of Genetic Resources of Plants, Animals and Microorganisms Important for Nutrition and Agriculture (NPGZM). The collection is located at the Research Institute of Dairy in Tábor (MILCOM a. s. company premises) and deals with the preservation of strains of bacteria, yeasts and filamentous fungi contaminating dairy and bakery products, which due to their properties have the potential for use in further research.
In the first year (2019), about 50 contaminants isolated from dairy products and dairies were included in the collection. Over the next two years, the collection expanded by another 29 contaminating strains. In the future, the collection will be expanded by isolates from bakery operations, especially from various yeasts, in accordance with the solution of NAZV projects, which are focused on antifungal activity and functional and technological properties of microorganisms in dairy products and yeasts.

• Collection characteristics, numbers of cultures
In 2021, 23 strains occurring as contaminants in the dairy and bakery industries were deposited in the bacterial part of the collection. These are bacteria from various types of cheese, milk, and dairies.
A large group of bacterial contaminants belongs to the genus Bacillus. These gram-positive sporulating rods form very resistant endospores allowing it to survive high temperatures, the effect of disinfectants, etc. Thus, can be very problematic in operations. Other representatives of spore-forming bacteria include, for example, Clostridium butyricum, which is known to produce gases and butyric acid in dairy products and cheese blowing. Representatives of the genus Staphylococcus are a frequent contaminant of dairy products, especially from unpasteurized milk. Bacteria of the genus Pseudomonas grow at relatively low (cold) temperatures (3-7 ° C) and produce undesirable lipases and proteases in milk and dairy products. Other representatives of deposited bacterial contaminants belong to the genera Psychrobacter, Kurtia, Kocuria, Corynebacterium, Lactococcus and Macrococcus.

• Strain storage methods
The collection uses two storage methods: lyophilization and cryopreservation. The lyophilization is used for long-term deposition of cultures and is performed externally in the central laboratory of NPGZM at the VÚRV v.v.i. A recovery interval is 3-5 years. Alternatively, the cryopreservation is used. The recovery interval is 5 years.

• Quality systems, internal standards, good laboratory practices
The CCDBC has fully taken over the ISO 9001 quality management system, which was introduced at the workplace where the collection is located, in 2002 as part of the Collection of dairy microorganisms - Laktoflora®. The activities of the collection are further ensured in accordance with the internal guidelines. All storage methods are described in managed documents.

• Services
all the strains of the CCDBC collection are provided free of charge for the teaching and research institution in accordance with the national program (NPGZM)
the service of isolation, cultivation and identification of strains is provided
professional advisory and consulting activities

• Inclusion in national and international organizations
The recently established CCDBC collection is part of the National Program for the Conservation and Utilization of Genetic Resources of Plants, Animals and Microorganisms Important for Food and Agriculture (NPGZM). It is not yet integrated into any international system.

• Contact:
Culture Collection of Dairy and Bakery Contaminants
MILCOM a.s., Soběslavská 841, 390 02 Tábor, Czech Republic
Tel.: +420 733 591 080, www.ccdm.cz

Ing. Miloslava Kavková, Ph.D., the head of the collection CCDBC
m.kavkova@vum-tabor.cz
+420 723 679 012
Ing. Zuzana Dlouhá, the curator of the collection CCDBC
z.dlouha@vum-tabor.cz
+420 733 591 080